Only if you plan to overclock the polling rate of a mouse. If you are using an old mouse like the wmo or ime 3.0 you may need to overclock the polling rate. The only other reason to use one over the other is if your hardware is faulty in which case you would use the USB port that is not faulty and disable the faulty one. That is all I can think of. It really shouldn't matter.

From my understanding, USB 3.0 doesn't support higher than 125 Hz. So it doesn't matter if you overclock too 500 Hz yourself of you have a mouse running 500 Hz out of the box, the USB won't poll higher than 125 Hz.

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No.The driver people commonly use to overclock the USB polling rate is not compatible with USB 3.0 ports. USB 3.0 ports work just like USB 2.0 ports when you plug a mouse into them.

I will answer again to be completely clear if your mouse has 500hz polling rate like the g100s when you plug into a USB 3.0 port it will be 500hz. And if you purchase a mouse that you use software to change the polling rate like steelseries or razer when plugged into a USB 3.0 port you can set them to 500hz and 1000hz just like you could with USB 2.0. But the method of overclocking your USB port on this site http://www.ngohq.com/news/15043-how-to-increa...a-7-a.html will not work with USB 3.0. I should also mention it will not work for windows 8 or 8.1. Sorry for the long post I j u wanted to make sure no one reading this got confused.

Both should work just the same. If one feels better, use it. But both support 1000 Hz with no problem.

Not exactly the same. USB 3.0 doesn't poll when idle. Check in latency monitor, ISR count will not update when you don't type on a USB keyboard or don't move your mouse when it's plugged into a USB 3.0 port.

On a USB 2.0 port, ISR count will just keep going and going.

So in essence the only benefit you stand to gain is less powerconsumption when idle. Perhaps interesting for those on a laptop.

Yes this is true. Also there should be no interrupts from a USB 3.0 port but this shouldn't make a difference.

I suspect that the not polling on idle is the same for all USB and depends on the OS. Have you tested both?

The thing is that the vast majority, if not all mice on the market are most likely going to be identified as USB 2.0 devices, and due to USB's backwards compatibility will behave according to the appropriate spec, which would be 2.0, even when plugged into a 3.0 port.